Fungal endophytes in spotted knapweed influence its competitive interactions

Fungal endophytes in spotted knapweed influence its competitive interactions. Abstract Fungal symbionts are often overlooked in studies of plant invasion. Nevertheless, their role could be essential to the competitive success of the invader. We studied fungal endophytes in the widespread invasive Centaurea stoebe (common knapweed). A preliminary experiment showed that endophytes in roots of C. stoebe significantly reduced the biomass of evolutionarily naïve neighbours (Festuca idahoensis), compared to endophyte-free C. stoebe. In the main experiment non-clavicipitaceous endophytes belonging to six phylotypes, were employed as root inoculants. Each of these endophytes again reduced the growth of naïve neighbours (F. idahoensis); and remarkably, each also increased the growth of adapted neighbours (F. ovina) that were tested for the first time. Four of the six endophytes caused C. stoebe to gain a competitive advantage over its naïve neighbour that was significantly greater than the endophyte-free C. stoebe over that same neighbour. However, endophyte-free C. stoebe had no greater competitive advantage over F. idahoensis than it had over F. ovina. Therefore, plant-plant interactions were dramatically affected by the presence of endophytes in a way that would favor invasion.